DreamTaq™ Green PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq™ DNA Polymerase, optimized DreamTaq™ Green buffer, MgCl2 and dNTPs. The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of PCR products on a gel. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion. The master mix retains all features of DreamTaq™ DNA polymerase. It is capable of robust amplification of up to 6 kb from genomic DNA and up to 20 kb from viral DNA. For applications that require PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless DreamTaq™ PCR Master Mix (2X). DreamTaq™ DNA polymerase incorporates modified nucleotides, but it is inhibited by dUTP.