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Oligo(dT)20 (50 µM), 50 µL
Random hexamers (50 ng/µL), 250 µL
5X RT buffer, 1 mL
0.1 M DTT, 250 µL
10 mM dNTP mix, 250 µL
SuperScript® IV RT (10,000 units total at 200 U/µl), 50 µL
RNaseOUT™ (40 U/µL), 100 µL
E. coli RNase H (2 U/µL), 50 µL
DEPC-treated water, 1.2 mL
Total HeLa RNA (10 ng/µL), 20 µL
Sense Control Primer (10 µM), 25 µL
Antisense Control Primer (10 µM), 25 µL
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