| Detail |
Kit for rapid mouse typing experiments with common samples such as tail clips, ear punch, hair, and blood
DNA preparation within 5 minutes
Loading dye included for subsequent gel electrophoresis
Designed for tail, ear, blood, and even hair samples
Technology - Simple sample preparation suitable for hot start PCR (optional direct PCR)
Format - Buffer and PCR mix (for 20–100 µL lysate and 10 µL PCR)
Sample material - Mouse tail clipping (1 mm), mouse ear punch (Ø 1 mm), mouse blood (1 μL), mouse hairs (approx. 3–30)
PCR - NucleoType HotStart PCR Master Mix for fast PCR genotyping (included)*
Amplicon size - Recommended for up to 1 kb
Preparation time - Lysis 2 min (18–25 °C); Enzyme inactivation 3 min (98 ° C);
PCR cycling - 30–90 min (cycler and target size dependent)
Stability - 12 months at 4 °C or -20 °C
*For amplicons < 1 kb an extension time of approx. 15 sec is recommended.
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