| Detail |
For 8–64 cDNA synthesis reactions: 5x RT SYBR Green Reaction Buffer, 5x RT Probe Reaction Buffer, 10x RT Enzyme Mix, UniSp6, RNA Spike-in template, RNase-Free Water
The miRCURY LNA RT Kit enables fast and convenient miRNA polyadenylation and reverse transcription in a single reaction step. Using one universal RT reaction as the template for subsequent PCR amplifications greatly simplifies the procedure compared with systems that require miRNA-specific first-strand synthesis, adding convenience and saving you time. The kit has been designed for use with the two miRCURY LNA miRNA PCR Systems, using either SYBR Green or an LNA-enhanced hydrolysis probe as the detection method. The kit contains two separate reaction buffer tubes for the respective PCR detection method.
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