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The QIAcuity OneStep Advanced EG PCR Kit enables sensitive quantification of RNA or RNA+DNA targets in one combined RT-PCR reaction on the QIAcuity Digital PCR System. The kit contains a 4x concentrated Master Mix and a 100x concentrated RT Mix optimized for microfluidic use in the QIAcuity Nanoplates. The kit improves the specificity and efficiency of dye-based digital PCR to provide accurate quantification analysis. As an intercalating dye, EvaGreen binds to double-stranded DNA and enhances the quantitative accuracy of gDNA or cDNA measurements on the QIAcuity dPCR instruments. The new kit is equipped with a HotStart Reverse Transcription (RT) Enzyme. Thanks to the RT Enzyme, you can work at room temperature to simultaneously set up your reaction and nanoplate for loading into QIAcuity Four or Eight instruments. The kit works in combination with the QIAcuity Digital PCR System and the QIAcuity Nanoplates.
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