Contains Earle's salts, L-glutamine, and 2,200 mg/L sodium bicarbonate.
Media 199 were originally formulated for nutritional studies of chick embryo fibroblasts.
Media utilizing Earle's salts are buffered with a bicarbonate/carbonic acid system and will maintain pH in a CO2 incubator. Use of Earle's salts in atmospheric conditions will result in rapid rise in pH of the culture medium.