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Description TA Cloning® Kit (pCR2.1 vector [part #45-0046] + One Shot TOP10 Chemically Competent E. coli, 20rxns [part #C404003] + manual [part #25-0024]), 1 Kit
Detail

Efficient Cloning of Taq-Amplified PCR Products

Description:
TA Cloning® Kits are designed for cloning Taq-amplified PCR products directly from a PCR reaction using an overnight ligation step. The kits use a pCR® vector and yield >=80% recombinants with >=90% of the recombinants containing inserts. Some of the convenient features of the pCR® vectors are:

 

 

 

 

 

  • 3´-T overhangs for direct ligation of Taq-amplified PCR products
  • Choice of T7 (pCR®2.1) or T7 and Sp6 (pCR®II) promoters for in vitro RNA transcription and sequencing
  • Versatile polylinker with flanking EcoR I sites for easy excision of inserts
  • Choice of kanamycin or ampicillin selection
  • Easy blue/white colony screening
  • M13 forward and reverse primer sites for sequencing

    Contents and Storage:
    TA Cloning® Kits contain linearized pCR®2.1 or pCR®II vector, T4 DNA ligase, 10X ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Competent cell kits contain One Shot® Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot® E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

     

     

 

 

 

 

Sku K204001
Quantity 1
Price $946.53
Brand Invitrogen
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All prices in Canadian dollars