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Description TOPO TA Cloning® Kit Dual Promoter (pCR®II-TOPO® vector [45-0640] + One Shot TOP10 Chemically Competent E. coli, 20rxns [44-0301 or C404003] + manual [25-0184] ), 1 Kit
Detail

Fast, Effective Cloning of Taq-amplified PCR Products

Description:
TOPO® TA Cloning® Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes. They use a pCR®-TOPO® Vector with covalently bound topoisomerase I for fast cloning and recombinants. pCR®-TOPO® Vectors include:

 

 

 

  • 3´-T overhangs for direct ligation of Taq-amplified PCR products
  • Choice of T7 (pCR®2.1-TOPO®) or T7 and SP6 (pCR®II-TOPO®) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
  • EcoR I sites flanking the PCR product insertion site for easy excision of inserts
  • Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants



    Contents and Storage:
    Each TOPO® TA Cloning® Kit contains linearized and topoisomerase I-activated pCR®2.1-TOPO® or pCR®II-TOPO® Vector, Salt Solution, dNTPs, Control Template and Primers, M13 Forward and Reverse Primers, One Shot® Chemically Competent or Electrocomp™ E. coli, S.O.C. Medium, and a supercoiled control plasmid. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

 

Sku K460001
Quantity 1
Price $894.06
Brand Invitrogen
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All prices in Canadian dollars