Sensitive Staining with Reduced Mutagenicity
SYBR Safe™ DNA Gel Stain provides sensitive DNA and RNA detection with substantially reduced mutagenicity, making it much safer to use than ethidium bromide. It is provided as a 10, 000X concentrate in DMSO, or as a convenient ready-to-use solution in 0.5X TBE buffer or 1X TAE buffer. SYBR Safe™ can be used either for post-staining or incorporated into gels. The detection sensitivity with SYBR Safe™ is comparable to that obtained with ethidium bromide. DNA bands stained with SYBR Safe™ DNA Gel Stain can be detected using a blue light transilluminator such as Invitrogen's Safe Imager™, a standard UV transilluminator, or a laser-based scanner. Bound to nucleic acids, the green-fluorescent SYBR Safe™ stain has fluorescence excitation maxima at ~280 and ~502 nm, and an emission maximum at ~530 nm.