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Ni Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification. Designed for scaling up purification of histidine-tagged proteins. High protein binding capacity and minimal leakage of Ni2+ ions. As a BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support. Available in convenient prepacked HisPrep and HisTrap column formats. Composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2+ ions for purification of histidine tagged proteins. The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2+ leakage while providing high capacity. Available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.ermediate purification.
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