For separation of cells, subcellular particles and larger viruses (down to ~70S) under gentle conditions which preserve viability and morphological integrity.
- Non-toxic to cells.
- Adjustable to physiological ionic strength and pH.
- Gradients can either be preformed or spontaneously generated by centrifugation at moderate speeds in an angle-head rotor.
- Gradients are iso-osmotic throughout and cover a range of densities up to 1.3 g/ml.