Features
Efficient - high yields of up to 20µg of high quality plasmid DNA.
Fast - procedure takes less than 14min.
Convenient - no phenol/chloroform extraction or alcohol precipitation required.
Effective - purified DNA is immediately ready to use.

Description
The GeneJET Plasmid Miniprep Kit provides a simple, rapid and cost-effective method for the isolation of high quality plasmid DNA from recombinant E.coli cultures. The kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20µg of high copy plasmid DNA per isolation procedure.

Principle
A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures such as conventional and fast digestion with restriction enzymes, PCR, transformation and automated sequencing.

Applications
Fast and easy isolation of high quality plasmid DNA suitable for all conventional molecular biology procedures, including:
- FastDigest restriction digestion;
- conventional restriction digestion;
- PCR, fast PCR;
- automated fluorescent sequencing;
- conventional radioactive sequencing;
- transformation;
- in vitro transcription.

Quality Control

The kit is tested for the isolation of pUC19 DNA from E.coli according to the protocol. The quality of isolated DNA is evaluated by spectrophotometric assay, agarose gel electrophoresis, digestion with restriction enzymes and automated fluorescent sequencing.

Components of the Kit

Resuspension Solution
Lysis Solution
Neutralization Solution
Wash Solution (concentrated)
RNase A
Elution Buffer
Spin Columns
Collection Tubes