For 25 x 50 ul reactions: QIAGEN OneStep RT-PCR Enzyme Mix (1 x 50 ul), 5x QIAGEN OneStep RT-PCR Buffer (1 x 250 ul), dNTP Mix (1 x 50 ul, 10 mM each), 5x Q-Solution (1 x 400 ul), RNase-free water (1 x 1.9 ml)
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