Highlights:

• Fast – obtain nuclear and cytoplasmic fractions in less than two hours
• Easy – eliminate freeze/thaw cycles, Dounce homogenization, lengthy centrifugation times and cold room work
• Versatile – obtain nuclear and cytoplasmic extracts separately from the same set of cells or tissue1,2
• Compatible – with downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays