Dilute protein samples 1:1 in 2x Laemmli sample buffer Laemmli sample buffer before loading on SDS-PAGE gels (requires the addition of β-mercaptoethanol).
-Formulation: 65.8 mM Tris-HCl, pH 6.8, 2.1% SDS, 26.3% (w/v) glycerol, 0.01% bromophenol blue
-Ready to dilute — use distilled deionized water
-Premixed loading buffer — ensures lane-to-lane consistency
-Quality-controlled reagent — guarantees reproducible results