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Description Phusion® High-Fidelity DNA Polymerase, 500 U (Replacement for EP0502)



The unique Phusion® DNA Polymerase offers performance that no other enzyme can match. The Phusion DNA Polymerase generates templates with an accuracy and speed unattainable with a single enzyme, even on your most difficult templates. The unique structure and characteristics of Phusion DNA Polymerase make it a superior choice for cloning, and sets a new standard for PCR performance: its error rate is 50-fold lower than that of Thermus aquaticus, and 6-fold lower than that of Pyrococcus furiosus - thus being the most accurate of available thermostable polymerases. Phusion DNA Polymerase possesses processivity 10-fold greater than Pyrococcus furiosus.


Phusion High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion® GC Buffer, DMSO and 50 mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer contain 7.5 mM MgCl2 in the provided 5x concentration. Storage buffer 20 mM Tris-HCl (pH 7.4 at 25 °C), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers, 200 µg/ml BSA and 50 % glycerol.

Storage and stability

Stable for 1 year from the assay date. Recommended storage temperature -20 °C.

Sku F530L
Quantity Available
Delivery 3 to 5 days
Price $506.76
Brand Thermo Scientific
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All prices in Canadian dollars