For 200 x 20 ul reactions: 4 x 100 ul 7x gDNA Wipeout Buffer, 4 x 50 ul Quantiscript Reverse Transcriptase, 4 x 200 ul 5x Quantiscript RT Buffer, 4 x 50 ul RT Primer Mix, 4 x 1.9 ml RNase-Free Water

For fast cDNA synthesis for sensitive real-time two-step RT-PCR:
cDNA synthesis and gDNA removal in only 20 minutes!
No need to design RNA-specific primers or probes!
High cDNA yields even from low-abundance transcripts!
cDNA synthesis from 5 and 3 regions of transcripts!