The QIAGEN One-Step RT-PCR Kit provides a blend of Sensiscript and Omniscript Reverse Transcriptases, HotStarTaq DNA Polymerase, QIAGEN OneStep RT-PCR Buffer, a dNTP mix, and Q-Solution, a novel additive that enables efficient amplification of "difficult" (e.g., GC-rich) templates. The easy one-tube setup and optimized components result in highly sensitive and successful results.

For 100 x 50 ul reactions: QIAGEN OneStep RT-PCR Enzyme Mix (1 x 200 ul), 5x QIAGEN OneStep RT-PCR Buffer (1 x 1 ml), dNTP Mix (1 x 200 ul, 10 mM each), 5x Q-Solution (1 x 2 ml), RNase-free water (2 x 1.9 ml)

For highly sensitive and successful one-step RT-PCR:
Fast and easy one-tube setup!
One-step RT-PCR of any RNA template without optimization!
Unique enzyme mix for high specificity and sensitivity!
Optimized reverse-transcription and amplification buffer!