The miRNeasy Mini Kit enables purification of total RNA which includes RNA from approximately 18 nucleotides (nt) upwards from all types of animal tissues and cells, including difficult-to-lyse tissues. Alternatively, an miRNA-enriched fraction and a total RNA (>200 nt) fraction can be purified separately (for separate purification, an RNeasy MinElute Cleanup Kit is required). The miRNeasy Mini Kit is used for low-throughput RNA purification using spin columns. Copurification of miRNA and total RNA using the miRNeasy Mini Kit can be automated on the QIAcube. For 50 preps: 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-Free Reagents and Buffers For purification of microRNA and total RNA from tissues and cells: Effective purification of miRNA and total RNA! Efficient enrichment of miRNA and RNAs <200 nucleotides! High-purity RNA suitable for all downstream applications! Protocols for copurification or isolation of separate fractions!
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