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The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least 8 orders of magnitude of enzyme concentration, and the patented technology incorporated into this assay system allows measurement of less than 10(-20) moles of luciferase under optimal conditions. Generally, 100-fold greater sensitivity can be achieved over chloramphenicol acetyltransferase (CAT) assays. The Luciferase Assay Reagent generates light that is nearly constant for at least 1 minute and so is compatible with measuring firefly luciferase in a single-tube luminometer or in a multiwell plate luminometer with an auto-injector. The Luciferase Assay System is a nonhomogeneous assay system; the cells containing the luciferase must be lysed before reagent addition.
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