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GoTaq DNA Polymerase is a proprietary formulation that gives robust amplification equal to and in some cases superior to that of standard Taq DNA polymerase. The supplied 5X GoTaq Green and Colorless Reaction Buffers contain MgCl2 at a concentration of 7.5mM for a final concentration of 1.5mM in the 1X reaction; they also contain a compound that increases sample density, so samples sink easily into wells of an agarose gel. These buffers allow you to go directly from thermal cycler to gel analysis. The green buffer also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis. The blue dye comigrates at the same rate as 3-5kb DNA fragments in a 1% agarose gel. The yellow dye migrates ahead of primers (<50bp). Use the green reaction buffer for direct-to-gel analysis after amplification and the colorless reaction buffer for post-amplification analysis by fluorescence or absorbance without prior DNA purification.
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