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RNasin Plus RNase Inhibitor is a recombinant mammalian RNase inhibitor expressed as a soluble protein in E. coli, allowing easy purification through a combination of ion exchange and hydrophobic interaction chromatography. Capable of inhibiting eukaryotic RNases and compatible with enzymes such as AMV, M-MLV and ImProm-II Reverse Transcriptases or Taq and Tfl DNA Polymerases, RNasin Plus offers increased resistance to oxidation over the human version of the protein. In addition, the new protein demonstrates continued inhibition of RNases above 50C. Heating solutions of RNasin Plus and RNase followed by cooling does not result in the reappearance of RNase activityeven when the solution is heated above the denaturation temperature of the RNasin Plus protein alone. This allows protection prior to, during and after heating, even at temperatures normally used during first-strand DNA synthesis in RT-PCR. Solutions heated up to 70C for 15 minutes did not result in RNase reactivation.
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