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Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ULK1 is a serine/threonine protein kinase that plays a critical role during the initial stages of autophagy. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13; this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner. Kinase: ULK1, 10ug (Human, recombinant; amino acids 1-649). MW: ~125kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Reaction Buffer, DTT. ULK1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/8408/.
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