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Full-length rhSYK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. SYK is a non-receptor protein tyrosine kinase that is widely expressed in hematopoietic cells. It is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation and phagocytosis. In B cells, SYK plays a crucial role in intracellular signal transduction induced by oxidative stress as well as antigen receptor engagement. SYK has been shown to act as a potential tumor suppressor in breast cancer. Absence of SYK protein in primary breast tumors is correlated with poor outcomes. SYK-deficient cells have increased motility that is restored to normalcy by replacement with wild-type SYK. Kinase Enzyme System contains: SYK, 10ug (Human, recombinant full-length). MW: ~100kDa. Substrate: Poly (4:1 Glu, Tyr) Peptide. Other: Reaction Buffer, DTT. SYK NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/6850/.
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