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Polyclonal secondary antibodies raised in goat, immunoaffinity-purified using immobilized antigens and conjugated to horseradish peroxidase (HRP) enzyme. The Anti-Mouse IgG (H+L), HRP Conjugate, antibody binds to both heavy and light chains for all IgG subclasses. As with all antibodies, in certain applications some species-dependent antigen-dependent cross-reactivity may be observed. The product (unless otherwise noted) is supplied as a 1mg/ml solution. A starting working dilution of 1:2,500 is suggested for most Western blot, dot blot and ELISA applications. The optimum concentration of secondary antibody depends on the application and will need to be empirically determined.
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