The REPLI-g Mini Kit provides optimized reagents for whole genome amplification (WGA) from small samples using innovative Multiple Displacement Amplification (MDA) technology. The typical DNA yield of a 50 μl reaction is up to 10 μg, with an average product length greater than 10 kb (ranging between 2 kb and 100 kb). Unique REPLI-g technology delivers highly uniform WGA from a variety of small or precious sample types, including purified genomic DNA, or directly from fresh or dried blood, buccal swabs, fresh or frozen tissue, and cells. This simple and reliable method is capable of accurate and unbiased amplification of genomes and generates DNA that can be applied without further purification or quantification for downstream applications that do not require labeling. In contrast to PCR-based WGA technologies, high fidelity rates are increased up to 1000-fold, avoiding costly false positive or negative results.

DNA Polymerase, Buffers, and Reagents for 100 x 50 µl whole genome amplification reactions (typical yield 10 µg per reaction)