Ideal for a wide variety of protein-analysis applications, these membranes with 0.2µm pore sizes feature naturally hydrophobic polyvinylidene fluoride filter media. The FluoroTrans® media are white, microporous solid phase supports that bind proteins tenaciously via hydrophobic interactions. The high surface area allows for strong hydrophobic interaction with proteins and typically adsorbs 50% more protein than nylon or nitrocellulose. Filter membranes are resistant to a wide range of chemical solvents (i.e., acetone, DMSO, dimethyl formamide) and will not shrink during destaining with methanol. Resistance to trifluoroacetic acid and triethylamine allows direct insertion of FluoroTrans® membrane materials into amino acid analyzers and gas phase protein sequencing equipment.

The FluoroTrans® PVDF membrane is optimized for N-terminal protein sequencing and features good signal-to-noise with standard detection systems. Immobilized proteins can be used directly for sequencing or visually detected with common staining reagents, including Amido Black, Ponceau S, and colloidal gold.

The FluoroTrans® W membrane is optimized for Western transfer applications but can also be used for Southern transfers. This membrane allows for sensitive protein detection with low background and very low protein burn-through. Immobilized proteins can be visually detected with Coomassie blue, Amido Black, Ponceau S, and colloidal gold.