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Recombinant human WNK1 (181-507) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. WNK1 is a member of the WNK subfamily of serine/threonine protein kinases that is a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in WNK1 have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. WNK1 is a regulator of blood pressure and deficiency of this protein in mice lowers the blood pressure (1). WNK1 can regulate the Ca2+-sensing and the subsequent Ca2+-dependent interactions mediated by synaptotagmin C2 domains, and WNK1 exhibits additive CFTR inhibition. Kinase Enzyme System contains: Kinase: WNK1, 10microg (Human, recombinant; amino acids 181-507). MW: ~67kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, MnCl2, DTT. WNK1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/10114/.
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