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100 x 20 µl reactions, 1 ml (1 x 1 ml) 2x one-step reaction mix, containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water. The iTaq universal probes one-step kit is a fast and convenient solution for real-time PCR using the powerful combination of iScript™ RNase H+ MMLV reverse transcriptase and antibody hot-start iTaq DNA polymerase in one reaction
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