Sodium Bicarbonate is buffer commonly used for maintaining the pH of cell culture medium in the presence of 4–10% carbon dioxide. In addition to buffering, sodium bicarbonate provides some nutritional benefit, while rarely displaying any cell toxicity. Formulated as 75 g per liter of water, Gibco® Sodium Bicarbonate, 7.5% Solution is perfect for supplementing dry powder medium during reconstitution. The final Sodium Bicarbonate concentration used in the medium depends on the media formulation and the carbon dioxide concentration used in the incubator.

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