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The UDP-Glo Glycosyltransferase Assay is a homogeneous, single-reagent-addition method to rapidly detect UDP formation in glycosyltransferase reactions. After the reaction, add an equal volume of UDP Detection Reagent to simultaneously convert the UDP product to ATP, generating light in a luciferase reaction. Detect light generated using a luminometer. Correlate luminescence to UDP concentration with a UDP standard curve. This assay is intended for use with purified glycosyltransferases that use UDP-sugar as a donor substrate and cannot be used with whole cells or cell extract. However, glycosyltransferases can be purified from cell extract using immunoprecipitation or affinity tag pull down before use in this assay. Note: The kits have changed from their original component configuration. The UDP-Glo Solution was replaced with UDP-Glo Enzyme and Enzyme Dilution Buffer. Also, note the change in the kit storage temperature from -20C to <=65C.
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