• 3 μl or 5 μl per loading for clear visualization during electrophoresis
on 15-well or 10-well mini-gel, respectively.
• 1.5~2.5 μl per well for general Western transferring.
• Apply more for thicker (> 1.5 mm) or larger gel.
The BLUelf Prestained Protein Ladder is a three-color protein standard
with 13 pre-stained proteins covering a wide range molecular weights
from 3.5 to 245 kDa. Proteins are covalently coupled with a blue
chromophore except for two reference bands (one green and one red
band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE
The BLUelf Prestained Protein Ladder is designed for monitoring protein
separation during SDS-polyacrylamide gel electrophoresis, verification
of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose)
and for approximating the size of proteins.
The ladder is supplied in gel loading buffer and is ready to use. Do not
heat, dilute, add reducing agent before loading.
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM
Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 10 mM Dithiothreitol, 3.6 M
Urea, and 15 % (v/v) Glycerol).