• 3 μl or 5 μl per loading for clear visualization during electrophoresis

on 15-well or 10-well mini-gel, respectively.

• 1.5~2.5 μl per well for general Western transferring.

• Apply more for thicker (> 1.5 mm) or larger gel.

The BLUelf Prestained Protein Ladder is a three-color protein standard

with 13 pre-stained proteins covering a wide range molecular weights

from 3.5 to 245 kDa. Proteins are covalently coupled with a blue

chromophore except for two reference bands (one green and one red

band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE

(Tris-glycine buffer).

The BLUelf Prestained Protein Ladder is designed for monitoring protein

separation during SDS-polyacrylamide gel electrophoresis, verification

of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose)

and for approximating the size of proteins.

The ladder is supplied in gel loading buffer and is ready to use. Do not

heat, dilute, add reducing agent before loading.

Contents

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM

Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 10 mM Dithiothreitol, 3.6 M

Urea, and 15 % (v/v) Glycerol).