| Detail |
For one-step qRT-PCR using SYBR Green I for gene expression analysis. For 500 x 20 µl reactions: 3 x 1.7 ml QuantiNova SYBR Green RT-PCR Master Mix, 100 µl QuantiNova SYBR Green RT Mix, 100 µl Internal Control RNA, 500 µl Yellow Template Dilution Buffer, 1 ml ROX Reference Dye, 2 x 1.9 µl RNase-Free Water. •Unique two-phase hot-start procedure for room-temperature setup
•Visual pipetting control, resulting in fewer pipetting errors
•Internal control for positive in-process verification of successful RT-PCR
•Accurate quantification over several logs of template
•Ultrafast, universal protocol for all standard and fast cyclers
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