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The NanoLuc Kinase Fusion Vectors contains the coding region of the indicated human kinase fused to either the C- or the N-terminus of NanoLuc luciferase. The vectors are designed for use with the NanoBRET Target Engagement (TE) Intracellular Kinase Assay, either K-4 or K-5, as indicated, where the plasmid can be transfected into various cell lines to enable kinase target engagement analysis.The NanoLuc luciferase kinase fusion vectors are supplied transfection-ready for introduction into a variety of cell lines. The CMV promoter drives the expression of the full-length NanoLuc kinase fusion protein. The placement of NanoLuc either 5 or 3 of the kinase gene is optimized for NanoBRET TE Kinase Assays. This orientation is reflected in the vector name. For example, NanoLuc-kinase implies NanoLuc is 5 to the kinase gene (at the N-terminus of the kinase). Similarly, kinase-NanoLuc implies NanoLuc is 3 to the kinase gene (at the C-terminus of the kinase).
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