| Detail |
The TIM-3 Bioassay (Cat.# JA2211, JA2215) is a bioluminescent reporter cell-based assay to measure the potency and stability of antibodies and other biologics targeting TIM-3. The assay consists of two cell lines: TIM-3 Effector Cells, Human T cells genetically engineered to express human TIM-3 and a NanoLuc luciferase reporter driven by T cell activation pathway-dependent response elements; TIM-3 Target Cells, MHCII-positive human cell line. The TIM-3 Effector and Target Cells are provided in thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell culture and propagation. When the two cell types are co-cultured, TIM-3 Target Cells provide low-level stimulation to the TIM-3 Effector Cells through the TCR. TIM-3 is engaged and activated by its ligands, including PS, which is present on both Effector and Target cells. The combined stimulation of the TCR and TIM-3 induces promoter-mediated luciferase activation and luminescence.
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